Active GMP Recombinant Human IL12A Protein

• Cat.No.: IL12A-129HG
• Product Overview: Recombinant Human IL12B(Ile23–Ser328) and Human IL12A(Arg23–Ser219), was co-produced in Sf 21 (baculovirus)-derived under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
• Molecular Mass: Predicted Molecular Mass: 34.7 (p40) & 22.5 (p35) kDa
  SDS-PAGE: 41 kDa (p40) and 29 kDa (p35), reducing conditions
• N-terminal Sequence Analysis: Ile-Trp-Glu-Leu-Lys-Lys-Asp-Val-Tyr-Val (p40)
  Arg-Asn-Leu-Pro-Val-Ala-Thr-Pro-Asp-Pro (p35)
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]
• Official Symbol: IL12A
• Synonyms: IL12A; NKSF1; interleukin-12 subunit alpha; CLMF; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; 35 kD subunit; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; P35; IL-12A
• Gene ID: 3592
• mRNA Refseq: NM_000882
• Protein Refseq: NP_000873
• MIM: 161560
• UniProt ID: P29459