|Product Overview:||Recombinant Human IL15, Asn49-Ser162, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL.
The specific activity of recombinant human IL-15 is approximately 4.5 x 10^5 U/μg, which is calibrated against recombinant human IL-15 WHO Standard (NIBSC code: 95/554).
|Molecular Mass:||Predicted Molecular Mass: 12.5 kDa|
|N-terminal Sequence Analysis:||Asn-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||IL15 interleukin 15 [ Homo sapiens ]|
|Synonyms:||IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15;|
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