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GMP Recombinant Human IL16 protein

Cat.No.: IL16-4330HG
Product Overview: Recombinant Human IL16 was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Description: Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (130 a.a.) contains 130 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 7.0.
Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CD4+ T Lymphocytes is in a concentration range of 1.0-100 ng/ml.
Molecular Mass: Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 130amino acids.
AA Sequence: MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS
Endotoxin: Less than 1 EU/µg of rHuIL-16, 130 a.a. as determined by LAL method.
Purity: > 95 % by SDS-PAGE and HPLC analyses.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: IL16 interleukin 16 [ Homo sapiens (human) ]
Official Symbol: IL16
Synonyms: LCF; NIL16; PRIL16; prIL-16; Interleukin-16 isoform 2, Lymphocyte chemoattractant factor, LCF
Gene ID: 3603
mRNA Refseq: NM_172217.3
Protein Refseq: NP_757366.2
MIM: 603035
UniProt ID: Q14005

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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