| Description : |
Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (130 a.a.) contains 130 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 7.0. |
| Bio-activity : |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CD4+ T Lymphocytes is in a concentration range of 1.0-100 ng/ml. |
| Molecular Mass : |
Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 130amino acids. |
| AA Sequence : |
MPDLNSSTDSAASASAASDVSVEST AEATVCTVTLEKMSAGLGFSLEGGK GSLHGDKPLTINRIFKGAASEQSET VQPGDEILQLGGTAMQGLTRFEAWN IIKALPDGPVTIVIRRKSLQSKETT AAGDS |
| Endotoxin : |
Less than 1 EU/µg of rHuIL-16, 130 a.a. as determined by LAL method. |
| Purity : |
> 95 % by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
| Quality Statement : |
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
| Shipping : |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
