GMP Recombinant Human IL2 Protein

Cat.No.: IL2-122HG
Product Overview: Recombinant Human IL2, Ala21-Thr153, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Bio-activity: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.
Molecular Mass: Predicted Molecular Mass: 15.5 kDa
SDS-PAGE: 13 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Ala21-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys
Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human IL-2 confirms the predicted molecular mass of 15549 Da.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in 100 mM acetic acid
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: IL2 interleukin 2 [ Homo sapiens ]
Official Symbol: IL2
Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; IL-2; lymphokine;
Gene ID: 3558
mRNA Refseq: NM_000586
Protein Refseq: NP_000577
MIM: 147680
UniProt ID: P60568

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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