| Description : |
Human Interleukin-31 (IL-31) is a T-cell derived cytokine that shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. It signals through a receptor complex comprised of GPL (GP130-like, IL-31RA) and OSMR (Oncostatin M receptor). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Bio-activity : |
The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of IL31 can effectively induce STAT3 activation. |
| Molecular Mass : |
Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids. |
| AA Sequence : |
SHTLPVRLLRPSDDVQKIVEELQSL SKMLLKDVEEEKGVLVSQNYTLPCL SPDAQPPNNIHSPAIRAYLKTIRQL DNKSVIDEIIEHLDKLIFQDAPETN ISVPTDTHECKRFILTISQQFSECM DLALKSLTSGAQQATT |
| Endotoxin : |
Less than 1 EU/μg of the protein by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably des centigradecated. Upon reconsitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw centigrade centigradeles. |
| Reconstitution : |
Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
| Quality Statement : |
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
| Shipping : |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
