GMP Recombinant Human IL4 protein
Cat.No. : | IL4-112HG |
Product Overview : | Recombinant Human IL4 protein was expressed in Escherichia coli. This product is produced under cGMP guidelines. |
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Description : | Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The human IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds. The human IL-4 shares about 40% aa sequence identity with mouse/rat IL-4 and they are species-specific in their activities. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg. |
Molecular Mass : | Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
Protein length : | 129 |
AA Sequence : | HKCDITLQEIIKTLNSLTEQKTLCT ELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHR HKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYS KCSS |
Endotoxin : | Less than 0.01 EU/µg of rHuIL-4 GMP as determined by LAL method. |
Purity : | >98% by SDS-PAGE analysis. > 95 % |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | IL4 |
Official Symbol : | IL4 |
Synonyms : | IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1; |
Gene ID : | 3565 |
mRNA Refseq : | NM_000589 |
Protein Refseq : | NP_000580 |
MIM : | 147780 |
UniProt ID : | P05112 |
Products Types
◆ Recombinant Protein | ||
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Il4-4344R | Recombinant Rat Il4 Protein | +Inquiry |
IL4-80H | Recombinant Human IL4 Protein | +Inquiry |
IL4-178H | Active Recombinant Human IL4 Protein | +Inquiry |
IL4-0804H | Recombinant Human IL4 Protein (H25-S153), Tag Free | +Inquiry |
◆ Lysates | ||
IL4-001MCL | Recombinant Mouse IL4 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewBy testing immunogenicity in animal models, detect whether an immune response is induced.
The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.
This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.
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