GMP Recombinant Human IL6 protein
Cat.No. : | IL6-4321HG |
Product Overview : | Recombinant Human IL6 protein was expressed in Escherichia coli. This product is produced under cGMP guidelines. |
- Specification
- Gene Information
- Related Products
Description : | Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40% a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl. |
Bio-activity : | Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 10⁷ IU/mg. |
Molecular Mass : | Approximately 20.8 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids. |
Protein length : | 183 |
AA Sequence : | VPPGEDSKDVAAPHRQPLTSSERID KQIRYILDGISALRKETCNKSNMCE SSKEALAENNLNLPKMAEKDGCFQS GFNEETCLVKIITGLLEFEVYLEYL QNRFESSEEQARAVQMSTKVLIQFL QKKAKNLDAITTPDPTTNASLLTKL QAQNQWLQDMTTHLILRSFKEFLQS SLRALRQM |
Endotoxin : | Less than 0.01 EU/µg of rHuIL-6 GMP as determined by LAL method. |
Purity : | >98% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | IL6 |
Official Symbol : | IL6 |
Synonyms : | IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6; |
Gene ID : | 3569 |
mRNA Refseq : | NM_000600 |
Protein Refseq : | NP_000591 |
MIM : | 147620 |
UniProt ID : | P05231 |
Products Types
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe dosage and usage of Il6 will vary according to the disease, and generally require individualized regulation by the physician according to the disease situation.
The pharmacokinetic characteristics of Il6 mainly include drug absorption, distribution, metabolism, excretion and other information.
Recombinant Il6 protein has a unique therapeutic advantage in the treatment of multiple myeloma. It can promote the proliferation and differentiation of hematopoietic stem cells and inhibit the growth of tumor cells.
Il6 has specific therapeutic effects for certain diseases, such as rheumatoid arthritis, multiple myeloma and so on.
This protein is widely used in the treatment of rheumatoid arthritis, pneumonia, multiple myeloma, lymphoma, malignant tumors and other diseases.
The effects of Il6 on immune cells are mainly manifested as promoting cell proliferation and differentiation, regulating immune response, etc.
Customer Reviews (3)
Write a reviewAccurate localization and function within the cell.
The binding affinity and affinity constant with the target are high.
Can form a stable micellar structure.
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