|Product Overview:||Recombinant Human IL7, Asp26-His177, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 10^5 units/μg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).
|Molecular Mass:||Predicted Molecular Mass: 17 kDa|
|N-terminal Sequence Analysis:||Met-Asp-(Cys)-Asp-Ile-Glu-Gly-Lys-Asp-Gly|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||IL7 interleukin 7 [ Homo sapiens ]|
|Synonyms:||IL7; interleukin 7; interleukin-7; IL 7; IL-7;|
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