GMP Recombinant Human INHBA Protein

Cat.No.: INHBA-114HG
Product Overview: Recombinant Human INHBA, Gly311-Ser426, was produced in CHO cell under cGMP guidelines.
Source: CHO cell
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626).
Molecular Mass: Predicted Molecular Mass: 13 (monomer) kDa
SDS-PAGE: 14 kDa, reducing conditions
24 kDa, non-reducing conditions
N-terminal Sequence Analysis: Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
Official Symbol: INHBA
Synonyms: INHBA; inhibin, beta A;inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; EDF; FRP;
Gene ID: 3624
mRNA Refseq: NM_002192
Protein Refseq: NP_002183
MIM: 147290
UniProt ID: P08476

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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