GMP Recombinant Human KITLG Protein

Cat.No.: KITLG-138HG
Product Overview: Recombinant Human KITLG, Glu26-Ala189, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The specific activity of recombinant human SCF is approximately 564 units/μg, which is calibrated against recombinant human SCF WHO Standard (NIBSC code: 91/682).
Molecular Mass: Predicted Molecular Mass: 18.6 kDa
SDS-PAGE: 19 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Glu26-Gly-Ile-(Cys)-Arg-Asn-Arg-Val-Thr
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human SCF confirms the predicted molecular mass of 18589 Da.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: KITLG KIT ligand [ Homo sapiens ]
Official Symbol: KITLG
Synonyms: KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand
Gene ID: 4254
mRNA Refseq: NM_000899
Protein Refseq: NP_000890
MIM: 184745
UniProt ID: P21583

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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