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GMP Recombinant Human SHH Protein

Cat.No.: SHH-141HG
Product Overview: Recombinant Human SHH, Cys24-Gly197 (Cys24Ile-Ile), was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.
Molecular Mass: Predicted Molecular Mass: 20 kDa
SDS-PAGE: 22 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Ile-Ile-Gly25-Pro-Gly-Arg-Gly-Phe-Gly
Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: SHH sonic hedgehog [ Homo sapiens ]
Official Symbol: SHH
Synonyms: SHH; sonic hedgehog; sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3;
Gene ID: 6469
mRNA Refseq: NM_000193
Protein Refseq: NP_000184
MIM: 600725
UniProt ID: Q15465

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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