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Active GMP Recombinant Human TNF Protein

Cat.No. : TNF-106HG
Product Overview : Recombinant Human TNF, Val77-Leu233, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
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  • Gene Information
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Source : E. coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
Molecular Mass : Predicted Molecular Mass: 17 kDa
SDS-PAGE: 17 kDa, reducing conditions
N-terminal Sequence Analysis : Met-Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >97%, by SDS-PAGE with silver staining.
Storage : Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100-200 μg/mL in PBS.
Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha;
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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