GMP Recombinant Human TNF Protein

Cat.No.: TNF-106HG
Product Overview: Recombinant Human TNF, Val77-Leu233, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
Molecular Mass: Predicted Molecular Mass: 17 kDa
SDS-PAGE: 17 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100-200 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol: TNF
Synonyms: TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha;
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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