Active GMP Recombinant Human VEGFA Protein

• Cat.No.: VEGFA-102HG
• Product Overview: Recombinant Human VEGFA, Met1-Arg191, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in HCl.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL.
  The specific activity of recombinant human VEGF165 is approximately 1.7 x 10^3 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286).
• Molecular Mass: Predicted Molecular Mass: 19.2 (monomer) kDa
  SDS-PAGE: 19-21 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala27-Pro-Met-Ala-Glu-Gly-Gly-Gly-Gln-Asn
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS. Alternatively, reconstitute at 500 μg/mL in sterile 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692