"hERG" Related Products

hERG Fluorescence Polarization Assay Kit

Cat.No.: Kit-0939
Description: The hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the assay demonstrate a high correlation with those obtained from patch clamp techniques.
Storage: hERG Fluorescence Polarization Assay Kit
(Other materials are required separately; please refer to the protocol)Includes:
• hERG Membranes
• hERG Tracer Red
• hERG Buffers
• E-4031
• hERG Fluorescence Polarization Assay Protocol
• Certificate of Analysis
Store the hERG Membranes at -80°C. The Tracer Red, buffers, and E-4301 should be stored at -20°C.
Shipping: Dry Ice
Size: 1 kit
Materials Required but Not Supplied: Microplate Reader
Detection method: Fluorescent

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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