Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Activity Kits > HSP90β (C-Terminal Domain) TR-FRET Kit

HSP90β (C-Terminal Domain) TR-FRET Kit

"HSP90&beta" Related Products.
384 Reactions
The HSP90β (C-Terminal) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90β, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90β using a fluorescence reader.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
HSP90β (C-Terminal), Biotin Labeled: 10 µg; -80°C PPID, GST-tag: 10 µg; -80°C Tb donor: 20 µl; -20°C Dye-labeled acceptor: 20 µl; -20°C 3x HSP90 TR-FRET Assay Buffer: 4 ml; -20°C White, Nonbinding, low volume, microtiter plate: 1; Room temp.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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