"ChIP" Related Products

Mammalian Chromatin Extraction Kit

Cat.No.: Kit-0214
Product Overview: Mammalian Chromatin Extraction Kit is used for isolating chromatin or DNA-protein complex from mammalian cells or tissues.
Description: Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, the ChIP method with specific antibodies directly against various transcriptional factors is widely demanded. For performing ChIP, chromatin or DNA-protein complex in cells or tissues should be first isolated.
Applications: The Mammalian Chromatin Extraction Kit is suitable for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Target Species: Mammals
Usage: For research use only (RUO)
Storage: Upon receipt: (1) Protease Inhibitor cocktails at 4°C; (2) Store remaining components at room temperature.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: Check if any buffers contain salt precipitates before use. If so, shake the buffer until the salts are re-dissolve.
Kit Components: 10X Lysis Buffer 11 mlExtraction Buffer 11 mlChromatin Buffer 11 mlProtease Inhibitor Cocktails (1000X)* 110 µl* Spin the solution down to the bottom prior to use.
Compatible Sample Types: Cell, Tissue
Features & Benefits: Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 60 minutes.Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc.
Products Types

◆ Assay kits

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.