Native Agaricus bisporus lectin Protein, Biotinylated

Cat.No.: Lectin-1762A
Product Overview: This product is the Biotinylated Agaricus bisporus lectin and has sugar specificity against Gal(b-1,5)GalNAc (T disaccharide).
Description: Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits. This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine. Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms. Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity. ABL binds all blood groups and O-linked glycans of IgA subclasses. Simple mono- and disaccharides are only marginally inhibitory.
Source: Agaricus bisporus
Species: Agaricus bisporus
Form: 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.08% sodium azide.
Bio-activity: Sugar Specificity: Gal(b-1,3)GalNAc (T disaccharide)
Applications: Immunohistochemistry; Immunocytochemistry; Immunofluorescence; Blotting Applications; Elispot; ELISAs; Glycobiology
Storage: Refrigerate in the dark.
Concentration: 2 mg/ml
Synonyms: Lectin; ABL
UniProt ID: Q00022

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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