Native Aleuria Aurantia Lectin Protein, Agarose bound

Cat.No.: Lectin-1751A
Product Overview: This product is the Agarose bound Aleuria Aurantia Lectin and has sugar specificity against Fucose, Arabinose.
Description: This lectin is a dimer of two identical subunits of about 36 kDa each. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids
Source: Aleuria Aurantia
Species: Aleuria Aurantia
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide
Bio-activity: Binding Capacity: >4 mg of fucosyl glycoprotein/ml of gel
Molecular Mass: 72 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 100 mM L-fucose in buffered saline can be used.
3, After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage: Refrigerate at 4 centigrade. DO NOT FREEZE.
Concentration: 2 mg/ml of settled gel
Synonyms: Lectin; AAL
UniProt ID: P18891

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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