|Product Overview:||Activated protective antigen (PA 63) has been prepared by trypsinization of protective antigen followed by removal of the 20 kDa cleavage product. PA 63 forms an oligomer and binds to both lethal factor (LF) and edema factor (EF).|
|Description:||Protective antigen (PA) is the receptor binding component of anthrax toxin. The original 83 kDa protein is cleaved by cellular furin-like proteases to form an activated 63 kDa monomer, PA63. In this form, PA63 binds to other PA63 molecules forming a ring-shaped heptamer. PA63 also has binding sites specific for the enzymes LF and EF adjacent to the pore formed on the cell surface for translocation. PA should be used in approximately seven-fold excess along with either EF or LF to intoxicate cells.|
|Form:||Each vial, when reconstituted with 0.5 ml water, contains 0.5 mg of activated protective antigen from Bacillus anthracis in 10 mM bis-Tris propane, 0.1 M NaCl, pH 9.0 with 1.25% trehalose.|
|Molecular Mass:||63 kDa|
|Endotoxin:||The endotoxin content, determined using a kinetic chromagenic LAL assay, is 1.16 EU/mg.|
|Applications:||Tissue Culture Application: For tissue culture applications, medium containing glutamine must be fresh. Ammonium ion is released when glutamine breaks down, and may prevent acidification of the endosome thereby inhibiting translocation of LF or EF into the cytosol. A stable form of glutamine may be used.|
|Storage:||This product is packaged aseptically, lyophilized and sealed under vacuum. Store at 2-8 centigrade prior to reconstitution.|
|Concentration:||Protein concentration was determined by a modification of the method of Bradford, using bovine serum as the standard.|
|Reconstitution:||Anthrax toxin proteins, when reconstituted in water, may be stored at 2-8 centigrade and used successfully within a few hours. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. Reconstitution with 1 mg/ml BSA will enhance stability and recovery. It is further recommended that the solution be aliquoted and frozen at either -20 centigrade or -80 centigrade. Avoid repeated freeze-thaw cycles. After the protein has been reconstituted as described above, cold glycerol may be added to 50% if a liquld is desired at freezer temperatures. Storage of material reconstituted with 1 mg/ml BSA at 2-8 centigrade for a period of two weeks may be acceptable for some applications.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.
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