Native Artocarpus integrifolia Jacalin Protein, Fluorescein labeled

Cat.No.: Lectin-1794A
Product Overview: This product is the Fluorescein labeled Artocarpus integrifolia Jacalin and has sugar specificity against Galactose. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description: Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.
Source: Artocarpus integrifolia
Species: Artocarpus integrifolia
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: 800 mM galactose (S-9003) or 100 mM melibiose.
Molecular Mass: 66 kDa
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade. and centrifuge before use.
Concentration: 5 mg/ml active conjugate
Synonyms: Lectin; Jacalin

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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