Native B. pertussis LPS protein

Cat.No.: LPS-194
Product Overview: LPS was isolated from Bordetella pertussis 165, highly purified.
Description: LPS extracted from Bordetella pertussis is different in structure when compared with LPS from many species such as E. coli and S. minnesota. A major difference in this Bordetella endotoxin lies in the absence of an O polysaccharide or O antigen chain which, when present, forms the outermost domain of LPS. A non-repeating trisaccharide takes the place of the O antigen in some of the B. pertussis LPS population; another LPS congener lacks the trisaccharide. Two LPS congeners are resolved by electrophoresis; the slower migrating A band has the trisaccharide attached and the faster migrating B band does not. Due to the lack of an O antigen, B. pertussis LPS is referred to as rough LPS or as a lipooligosaccharide (LOS).
Source: B. pertussis 165
Species: B. pertussis 165
Form: LPS is dispersible in aqueous solvents at concentrations of 1 mg/ml. To achieve suspension in water, heating to about 50 centigrade with intermittent vortexing or sonication is recommedded. Allow ample tion for dispersion to occur. The use of 0.5% triethlamine aids in dispersion. Triehylamine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains. This LPS can be solubilized in % SDS in water.
Storage: It is recommended that this material be stored at 2-8 centigrade prior to and following reconstitution.
Concentration: 1 mg/mL
Warning: Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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