|Product Overview:||Native pertussis toxin was purified from Bordetella pertussis.|
|Description:||Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa).|
|Form:||83 µg/ml in 10% glycerol 10 mM phoshate buffer (pH 7.4), 137 mM NaCl|
|Bio-activity:||Cytotoxicity of the PT was confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT.|
|Molecular Mass:||PT consists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa).|
|Applications:||1. Studies on the signal transduction of the trimeric GTP binding proteins.
2. Therapeutic role in treating a number of diseases including hypertention, viral and autoimumune inhibitions.
3. Used for producing model mice for multiple sclerosis. http://www.jove.com/video/51275/myelin-oligodendrocyte-glycoprotein-mog35-55-induced-experimental.
|Notes:||The toxin can be inactivated by boiling for 30 min.|
|Storage:||Shipped with dery-ice and store at -80centigrade.|
|Warning:||It should be handled carefully by persons with expertise in knowledge and techniques for the safe handling of bacterial toxins. Avoid mouth pipetting. Wear protective gloves on handling the toxin. Avoid contact with open wounds. Wash thoroughly any area of the body that makes contact with the toxin. It is recommended that persons who handle the toxin are immunized by pertussis vaccine.|
|Gene Name:||Pertussis toxin subunit 1 [Bordetella pertussis Tohama I]|
|Synonyms:||ptxA; Pertussis toxin subunit 1|
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