|Product Overview:||Native toxin B purified from C. difficile strain VPI10463 (toxinotype 0).|
|Description:||Clostridium difficile Toxin A and B, cation-dependent UDP-glucose glucosyltransferases, are cellular toxins that inactivate Rho (and Rho family small GTPases) through monoglucosylation of these family members. Effects of this monoglucosylation include disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Rho proteins are monoglucosylated by Toxin A and B using UDP-glucose as a cosubstrate. Rho, Rac and Cdc42 are included in the Rho subfamilies targeted by both toxins. Low molecular mass GTP-binding proteins that are not modified by Toxin A and B include Ras, Rab, Arf, or Ran subfamilies as well as heterotrimeric G proteins.|
|Molecular Mass:||300 kDa|
|Storage:||Store at 2-8 centigrade|
|Reconstitution:||Contents of the 50UG sku when reconstituted in 250 μL sterile distilled water will contain Toxin B at a concentration of approximately 0.2 mg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose. Contents of the 2UG sku when reconstituted in 250μL sterile distilled water will contain Toxin B at a concentration of approximately 8 μg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.|
|Synonyms:||SKU; Clostridium difficile Toxin B|
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