Native Canavalia ensiformis Concanavalin A Protein, Biotinylated

Cat.No.: Lectin-1757C
Product Overview: This product is the Biotinylated Concanavalin A (Con A) and has sugar specificity against Galactose and Lactose. The excitation maximum is at 552 nm and the emission maximum is at 565 nm.
Description: Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.
Source: Canavalia ensiformis
Species: Canavalia ensiformis
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM Ca2+, 0.08% sodium azide, and a protein stabilizer.
Bio-activity: Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
Molecular Mass: 104 kDa
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade and centrifuge before use.
Concentration: 1 mg/ml active conjugate
Synonyms: Lectin; Con A
UniProt ID: P02866

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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