Native Diphtheria Toxin CRM197, Mutant [Glu52]
| Cat.No. : | CRM197-190 |
| Product Overview : | CRM197 was expressed in Corynebacterium diphtheriae, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose. Moisture: <3% by Karl-Fisher |
- Specification
- Gene Information
- Related Products
| Description : | The Glu52 diptheria toxin, also known as CRM197 (Cross-reactive materials 197), is a nontoxic mutant of diphtheria toxin, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. |
| Source : | Corynebacterium diphtheriae |
| Species : | Corynebacterium diphtheriae |
| Form : | Lyophilized powder (liquid also could be provided) |
| Molecular Mass : | ~58 kDa |
| Sterility : | Inoculation/membrane filtration |
| Endotoxin : | <10 EU/μg of protein by LAL method. |
| Purity : | > 90% by SDS-PAGE and HPLC |
| Storage : | Store at -20 centigrade. |
| Concentration : | 1~20mg/ml(aq) by BCA or Lowry |
| Reconstitution : | Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose. Vials containing appoximately 5mL of CRM197(aq) were lyophilized. The lyophilized powder can be reconstituted with water or sodium phosphate buffer. We could provide liquid or lyophilized powder. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket
