|Product Overview:||Shiga Like Toxin 2 (Verotoxin 2) protein was purified from E. coli O157.|
|Description:||Shiga toxin is a group of related toxins produced by Shigella dysenteriae and enterohemorrhagic Escherichia coli (EHEC). In the past, shiga toxins have been differentiated as Shiga, Shiga-like or vero toxins but since the toxins produced by the two types of bacteria are very similar, distinctions are no longer made.
Shiga toxin is produced by specific strains of Escherichia coli in two isoforms, Shiga 1(Stx1) and Shiga 2(Stx2) which share ~60% sequence homology. These Shiga toxins have the same molecular structures, enzymatic activities, receptor specificity and intracellular trafficking process. Shiga toxins are composed of two subunits, an enzymatically active A subunit which is non-covalently attached to the pentameric B subunit, responsible for binding to the target cells. The sugar domain of the glycosphingolipid, globotriaosylceramide, Gb3, located on cell surfaces, is specifically bound by the B-subunit. The shiga toxin is internalized by endocytosis and is transported via a retrograde pathway in the Golgi apparatus to the endoplasmic reticulum. The enzymatic A subunit is then translocated to the cytosol where its N-glycosidase activity irreversibly modifies ribosomal 28S RNA leading to inhibition of protein synthesis and causing cell death. The potential use of Shiga toxin to induce apoptosis in cancer cells is currently an active area of research.
|Source:||E. coli O157|
|Species:||E. coli O157|
|Form:||When reconstituted is in 0.01 M Tris ph 8.6 and 0.001 M Nacl, 0.001% poluvinyl alcohol.|
|Storage:||Store frozen at -80 centigrade. Avoid repeated freeze and thaw cycles.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.|
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