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Native E. coli Shiga Toxin 2 protein

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By Species
E. coli(1)
By Source
E. coli(1)
Cat.No.:
Shiga Toxin 2-209
Product Overview:
Shiga Toxin 2 was purified from Escherichia coli.
Description:
Shiga toxin is a group of related toxins produced by Shigella dysenteriae and enterohemorrhagic Escherichia coli (EHEC). In the past, shiga toxins have been differentiated as Shiga, Shiga-like or vero toxins but since the toxins produced by the two types of bacteria are very similar, distinctions are no longer made.
Shiga toxin is produced by specific strains of Escherichia coli in two isoforms, Shiga 1(Stx1) and Shiga 2(Stx2) which share ~60% sequence homology. These Shiga toxins have the same molecular structures, enzymatic activities, receptor specificity and intracellular trafficking process. Shiga toxins are composed of two subunits, an enzymatically active A subunit which is non-covalently attached to the pentameric B subunit, responsible for binding to the target cells. The sugar domain of the glycosphingolipid, globotriaosylceramide, Gb3, located on cell surfaces, is specifically bound by the B-subunit. The shiga toxin is internalized by endocytosis and is transported via a retrograde pathway in the Golgi apparatus to the endoplasmic reticulum. The enzymatic A subunit is then translocated to the cytosol where its N-glycosidase activity irreversibly modifies ribosomal 28S RNA leading to inhibition of protein synthesis and causing cell death. The potential use of Shiga toxin to induce apoptosis in cancer cells is currently an active area of research.
Source:
E. coli
Species:
E. coli
Form:
When reconsitituted with 100 ul of wateris in 0.05M Tris, 0.1M Nacl, 0.1% Trehalose, PH 7.5. Use sufficient volume to wash down the walls and stopper, if necessary.
Molecular Mass:
This product migrates as two major bands corresponding to the A and B polypetides, with apparent molecular weights of 33 kDa and 8 kDa, respectively.
Endotoxin:
The endotoxin content, determined using a kinetic chromagenic LAL assay, is <90 EU/mg.
Purity:
>98%
Storage:
This product is supplied as a lyophilized powder, stoppered under vacuum. Store at 2-8 centigrade.
Warning:
Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Persons handling this product and contaminated glassware should consult the current version of the Biosafety in Microbiological and Biomedical Laboratories.
2. This product is to be used by skilled personnel under the direction of a principal investigator in a laboratory setting only.
3. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
4. Never remove the stopper prior to reconstitution and never work with the product in the powdered form. Always reconstitute it first.
5. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
6. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
7. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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