Native E.coli K12 LPS protein

Cat.No.: LPS-198
Product Overview: LPS purified was from scherichia coli K12 strain LCD25.
Description: Lipopolysaccharides (LPS), also known as lipoglycans and endotoxins, are large molecules consisting of a lipid and a polysaccharide composed of O-antigen, outer core and inner core joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, and elicit strong immune responses in animals.
Source: E. coli K12 strain LCD25
Species: E. coli K12 strain LCD25
Form: LPS is dispersible in aqueous solvents at concentrations of 1 mg/ml. To achieve suspension in water, heating to about 50 centigrade with intermittent vortexing or sonication is recommedded. Allow ample tion for dispersion to occur. The use of 0.5% triethlamine aids in dispersion. Triehylamine is very basic and may be neutralized with Tris HCl to avoid hydrolysis of the LPS fatty acid chains.
Storage: Store at 4 centigrade prior to and following reconstitution.
Concentration: 1 mg/mL
Warning: Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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