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Active Native Galanthus Nivalis Lectin Protein, Agarose bound

Cat.No. : Lectin-1776G
Product Overview : This product is the Agarose bound Galanthus Nivalis Lectin and has sugar specificity against Mannose.
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Description : Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca2+ or Mn2+ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.
Source : Galanthus nivalis
Species : Galanthus nivalis
Form : 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca , 20 mM mannose, 0.08% sodium azide, 0.01 mM Mn
Bio-activity : Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside
Molecular Mass : 50 kDa
Applications : Glycobiology, Affinity Chromatography
Usage : 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 0.1 M α methyl mannoside can be used. For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage : Refrigerate. DO NOT FREEZE.
Concentration : Protein Concentration: 3.0 mg/ml of settled gel
Synonyms : Lectin; GNL
UniProt ID : P30617
Synonyms : Lectin; GNL
UniProt ID : P30617

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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