Native Galanthus Nivalis Lectin Protein, Agarose bound

Cat.No.: Lectin-1776G
Product Overview: This product is the Agarose bound Galanthus Nivalis Lectin and has sugar specificity against Mannose.
Description: Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca2+ or Mn2+ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.
Source: Galanthus nivalis
Species: Galanthus nivalis
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca , 20 mM mannose, 0.08% sodium azide, 0.01 mM Mn
Bio-activity: Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside
Molecular Mass: 50 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 0.1 M α methyl mannoside can be used. For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage: Refrigerate. DO NOT FREEZE.
Concentration: Protein Concentration: 3.0 mg/ml of settled gel
Synonyms: Lectin; GNL
UniProt ID: P30617

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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