Native Galanthus Nivalis Lectin Protein, Biotinylated

Cat.No.: Lectin-1777G
Product Overview: This product is the Biotinylated Galanthus Nivalis Lectin and has sugar specificity against Mannose.
Description: Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca2+ or Mn2+ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.
Source: Galanthus nivalis
Species: Galanthus nivalis
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+.
Bio-activity: Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside
Molecular Mass: 50 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Storage: Refrigerate; for long term storage, aliquots may be stored frozen. If a precipitate forms upon long-term storage, warm to 37 centigrade. and centrifuge before use
Concentration: 2 mg/ml
Synonyms: Lectin; GNL
UniProt ID: P30617

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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