|Product Overview:||This product is the unconjugated Griffonia Simplicifolia Lectin I isolectin B4 and has sugar specificity against Galactose.|
|Description:||GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.|
|Bio-activity:||This product will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2 µg/ml. No agglutination of blood group A,
glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 100 µg/ml.
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
|Molecular Mass:||114 kDa|
|Applications:||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology|
|Storage:||Refrigerate in the dark.|
|Reconstitution:||Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended. For preserving solutions, stored at 4 centigrade, 0.08% sodium azide can be used.|
|Synonyms:||Lectin; GSL I; BSL I|
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