Native Griffonia Simplicifolia Lectin I isolectin B4 Protein, Biotinylated

Cat.No.: Lectin-1782G
Product Overview: This product is the Biotinylated Griffonia Simplicifolia Lectin I isolectin B4 and has sugar specificity against Galactose.
Description: GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.
Source: Griffonia Simplicifolia
Species: Griffonia Simplicifolia
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM
Bio-activity: This product will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 3 µg/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 100 µg/ml.
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
Molecular Mass: 114 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml.
Storage: Refrigerate; for long term storage, aliquots may be stored frozen.
Concentration: 0.5mg/ml
Synonyms: Lectin; GSL I; BSL I
UniProt ID: Q8W1R6

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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