Native Griffonia Simplicifolia Lectin II Protein, Fluorescein labeled

Cat.No.: Lectin-1789G
Product Overview: This product is the Fluorescein labeled Griffonia Simplicifolia Lectin II and has sugar specificity against N-Acetylglucosamine. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description: This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.
Source: Griffonia Simplicifolia
Species: Griffonia Simplicifolia
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine
Molecular Mass: 113 kDa
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark.
Concentration: 2.0 mg/ml
Synonyms: Lectin; GSL II; BSL II
UniProt ID: Q41263

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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