Native Hippeastrum Hybrid Lectin Protein, Biotinylated

Cat.No.: Lectin-1791H
Product Overview: This product is the Biotinylated Hippeastrum Hybrid (Amaryllis) Lectin and has sugar specificity against Mannose.
Description: This lectin is composed of four subunits of identical size and is immunologically related to GNL and NPL. Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans.
Source: Hippeastrum Hybrid
Species: Hippeastrum Hybrid
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Bio-activity: Inhibiting Sugar: 100 mM α-methylmannoside
Molecular Mass: 50 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml.
Storage: Refrigerate in the dark.
Concentration: 2 mg/ml
Synonyms: Lectin; HHL, AL

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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