| Description : |
Lens culinaris agglutinin is composed of four subunits - two of about 17 kDa and two of 8 kDa. LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A. |
| Source : |
Lens Culinaris |
| Species : |
Lens Culinaris |
| Form : |
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide, 0.1 mM Ca2+, 0.01 mM Mn2+ |
| Bio-activity : |
Carbohydrate-Binding Specificity: Mannose and Glucose |
| Molecular Mass : |
50 kDa |
| Applications : |
Immunofluorescence, Glycobiology |
| Usage : |
The recommended concentration range for use is 5-20 µg/ml. |
| Storage : |
Refrigerate in the dark. If precipitate forms upon long term storage, warm to 37 centigrade. A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade. and centrifuge before use. |
| Concentration : |
5 mg/ml |
| Synonyms : |
Lectin; LCA; LcH |
| UniProt ID : |
P02870 |
