Native Lens Culinaris Agglutinin Protein, Rhodamine labeled

Cat.No.: Lectin-1733L
Product Overview: This product is the Lens Culinaris Agglutinin and has sugar specificity against mannose and glucose. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
Description: Lens culinaris agglutinin is composed of four subunits - two of about 17 kDa and two of 8 kDa. LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.
Source: Lens Culinaris
Species: Lens Culinaris
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide, 0.1 mM Ca2+, 0.01 mM Mn2+
Bio-activity: Carbohydrate-Binding Specificity: Mannose and Glucose
Molecular Mass: 50 kDa
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark. If precipitate forms upon long term storage, warm to 37 centigrade. A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4 centigrade, warm to 37 centigrade. and centrifuge before use.
Concentration: 5 mg/ml
Synonyms: Lectin; LCA; LcH
UniProt ID: P02870

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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