Native Narcissus Pseudonarcissus (Daffodil) Lectin Protein

Cat.No.: Lectin-1743N
Product Overview: This product is the Hippeastrum Hybrid (Amaryllis) Lectin and Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures.
Description: NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.
Source: Narcissus Pseudonarcissus
Species: Narcissus Pseudonarcissus
Bio-activity: Inhibiting Sugar: 400 mM α-methylmannoside
Molecular Mass: 59 kDa
Applications: Glycobiology
Storage: Refrigerate in the dark.
Reconstitution: Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended. For preserving solutions stored at 4 centigrade, 0.08% sodium azide can be used.
Synonyms: Lectin; NPL, NPA
UniProt ID: Q40423

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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