| Description : |
Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection. |
| Source : |
Pisum Sativum |
| Species : |
Pisum Sativum |
| Bio-activity : |
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside |
| Molecular Mass : |
53 kDa |
| Applications : |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation |
| Storage : |
Refrigerate |
| Reconstitution : |
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended. For preserving solutions stored at 4 centigrade, 0.08% sodium azide can be used. |
| Synonyms : |
Lectin; PSA |
| UniProt ID : |
P02867 |
