| Description : |
Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection. |
| Source : |
Pisum Sativum |
| Species : |
Pisum Sativum |
| Form : |
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.01 mM Mn2+, 0.08% sodium azide |
| Bio-activity : |
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside |
| Molecular Mass : |
53 kDa |
| Applications : |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Usage : |
For most applications we recommend a freshly prepared working solution of 5-20 µg/ml. |
| Storage : |
Refrigerate; for long term storage, aliquot and store frozen. |
| Concentration : |
5 mg/ml |
| Synonyms : |
Lectin; PSA |
| UniProt ID : |
P02867 |
