Native Pisum Sativum Agglutinin Protein, Biotinylated

Cat.No.: Lectin-1828P
Product Overview: This product is the Biotinylated Pisum Sativum Agglutinin (PSA) and has sugar specificity against Mannose and Glucose.
Description: Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.
Source: Pisum Sativum
Species: Pisum Sativum
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.01 mM Mn2+, 0.08% sodium azide
Bio-activity: Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
Molecular Mass: 53 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Usage: For most applications we recommend a freshly prepared working solution of 5-20 µg/ml.
Storage: Refrigerate; for long term storage, aliquot and store frozen.
Concentration: 5 mg/ml
Synonyms: Lectin; PSA
UniProt ID: P02867

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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