Native Ricinus Communis Agglutinin II Protein

Cat.No.: Lectin-1834R
Product Overview: This product is the Unconjugated Ricinus Communis Agglutinin II (RCA II, RCA60, ricin) and has sugar specificity against Galactose, N-Acetylgalactosamine, and Lactose.
Description: Ricinus communis agglutinin II is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28 kDa and 32 kDa, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic.
Source: Ricinus Communis
Species: Ricinus Communis
Form: 10 mM phosphate, 0.15 M NaCl, pH 7.8, 0.08% sodium azide
Bio-activity: Inhibiting/Eluting Sugar: 200 mM galactose or lactose
Molecular Mass: 60 kDa
Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology
Usage: THIS IS A TOXIC LECTIN! EXERCISE CAUTION IN ITS USE! NEVER PIPET SOLUTIONS OF THIS LECTIN BY MOUTH. DO NOT LYOPHILIZE!
Storage: Store at 4 centigrade
Concentration: 5.0 mg/ml
Synonyms: Lectin; RCA II, RCA 60, ricin
UniProt ID: P02879

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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