|Product Overview:||Alpha Toxin is produced by Staphylococcus aureus strain Wood 46. Biological activity is assessed by measuring hemolysis of rabbit erythrocytes.|
|Description:||Alpha toxin, produced by Staphylococcus aureus, also known as alpha hemolysin, is one of many virulence factors produced by Staphylococcus aureus. Alpha Toxin is a single polypeptide chain having a molecular weight of 33,000 Daltons. Many cell types are sensitive to cytolysis by alpha toxin. Under controlled conditions, however, nucleated cells can be treated with alpha toxin without causing lysis. Under these conditions, alpha toxin monomers bind the cell membrane and form a heptamer which functions as a hydrophilic pore of diameter 1-3 nm. The molecular weight cut-off of material passing through the pore is approximately 2000 to 4000 Daltons and the pore is somewhat ion selective. The cell becomes permeablilized to small molecules, while large enzymes and carbohydrates are excluded. Alpha toxin has been utilized to introduce ions, substrates, inhibitors, nucleotides and other pharmacological agents into cells and thereby regulate cell physiology or function.|
|Form:||Each vial, when reconstituted with 500 ul water, the protein in 0.01 M sodium phosphate ph 7.2. Handle the product gently, do not vortex.|
|Endotoxin:||The endotoxin content, determined using a kinetic chromagenic LAL assay, is 5 EU/mg.|
|Storage:||This product is provided as an aseptically packaged lyophilized powder, sealed under vacuum. Store at 2-8 centigrade prior to and following reconstitution.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.|
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