"Enterotoxin Type B" Related Products

Native S. aureus Enterotoxin Type B protein

Cat.No.: Enterotoxin Type B-221
Product Overview: Enterotoxin Type B was produced in a native Staphylococcus aureus.
Description: Staphylococcal enterotoxins (SEs), produced by Staphylococcus aureus, are responsible for significant human disease including both food poisoning and toxic shock syndrome. Two of these enterotoxins, staphylococcal enterotoxins A and B (SEA and SEB) are described as superantigens for their ability to bind to Major Histocompatibility Complex molecules on antigen presenting cells and stimulate a large population of T cells, producing an inappropriate flood of cytokines. Although they are important participants in an immune response, cytokines, when overproduced, lead to toxic shock. Recently SEB has been found to bind to another regulator of the T cell immune response, CD 28. When added to human peripheral blood cells, SEB produces large quantities of IL-2, TNF-A and INF-c. SEs are resistant to heat, acid and proteases making them a common cause of food–borne disease.
Source: S. aureus
Species: S. aureus
Form: Each vial, when reconstituted with 500 ul water, the protein in 0.07 M sodium phosphate Ph 6.8. Handle the product gently; do not vortex.
Endotoxin: The endotoxin content, determined using a kinetic chromagenic LAL assay, is 14 EU/mg.
Purity: >95%
Notes: Care must be taken in the design of experiments with the toxoid because although toxicity has been reduced, sufficient toxicity may remain to cause death under certain conditions.
Storage: This product is provided as lyophilized powder, sealed under vacuum. Store at 2 - 8 centigrade prior to reconstitution. Following reconstitution, aliquot and freeze.
Concentration: 1 mg/mL
Warning: Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Persons handling this product and contaminated glassware should consult the current version of the Biosafety in Microbiological and Biomedical Laboratories.
2. This product is to be used by skilled personnel under the direction of a principal investigator in a laboratory setting only.
3. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
4. Never remove the stopper prior to reconstitution and never work with the product in the powdered form. Always reconstitute it first.
5. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
6. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
7. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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