Native Succinylated Wheat Germ Agglutinin Protein, Fluorescein labeled

Cat.No.: Lectin-1866W
Product Overview: This product is the Fluorescein labeled succinylated Wheat Germ Agglutinin and has sugar specificity against N-Acetylglucosamine. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Description: This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.
Source: Wheat germ
Species: Wheat germ
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate; or 500 mM N-acetylglucosamine with salt and/or acid elution generally required
Molecular Mass: 36 kDa
Applications: Immunofluorescence, Glycobiology
Usage: The recommended concentration range for use is 5-20 µg/ml.
Storage: Refrigerate in the dark; for long term storage, aliquots may be stored frozen.
Concentration: 5 mg/ml
Synonyms: Lectin; WGA; Succinylated WGA

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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