|Product Overview:||Toxin A was purified from Clostridium difficile.|
|Description:||A major nosocomial pathogen, Clostridium difficile causes antibiotic-associated colitis and intestinal Inflammatory disease, together called CDI. By releasing two large protein enterotoxins, C. difficile Toxin A (TcdA) and C. difficile Toxin B (TcdB), Clostridium difficile mediates inflammatory diarrhea and compromises intestinal epithelial cells. Either toxin when administered to cultured cells disrupts the cytoskeleton and sets off capsase-dependent apoptosis, although, C. difficile Toxin B is many times more potent than C. difficile Toxin A. Both toxins also activate intestinal epithelial and immune cells to produce cytokines and chemokines, likely contributing to the complications associated with severe CDI. Intracellular targets of TcdA and TcdB are RhoGTPases which regulate many host cell processes including establishing an epithelial barrier and the migration and signaling of immune cells. When the Rho proteins are glycosylated by C. difficile Toxins A or B, signaling is disrupted leading to the intestinal damage and inflamation characteristic of CDI.|
|Form:||50 mM NaCl, pH 7.5 and 0.1% trehalose. Handle the product gently. Do not votex.|
|Endotoxin:||The endotoxin content, determined using a kinetic chromagenic LAL assay, is <30 EU/mg.|
|Storage:||This product is packaged aseptically, lyophilized sealed under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved in water, this product is stable for up to 2 months when store at 2-8 centigrade.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.|
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