Native Ulex Europaeus Agglutinin I Protein, Agarose bound

Cat.No.: Lectin-1849U
Product Overview: This product is the Agarose bound Ulex Europaeus Agglutinin I and has sugar specificity against fucose and arabinose.
Description: Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.
Source: Ulex Europaeus
Species: Ulex Europaeus
Form: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide,
Bio-activity: Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose
Carbohydrate-Binding Specificity: Fucose and Arabinose
Molecular Mass: 63 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 100 mM L-fucose in buffered saline can be used.
3, After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage: Refrigerate. DO NOT FREEZE.
Concentration: 2 mg/ml of settled gel
Synonyms: Lectin; UEA I
UniProt ID: P22972

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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