Native Ulex europaeus Lectin, FITC conjugated

Cat.No.: Lectin-1715U
Product Overview: This product is the ulex europaeus lectin which againsts fucose blood group H. Affinity purified UEA I is conjugated with Fluorescein-5- isothiocyanate (FITC-isomer 1) and purified on Gel filtration column. A max. = 490 nm, E max =520 nm, Ratio of A490/A280= 1.0-1.4.
Description: The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R
Source: Ulex europaeus
Species: Ulex europaeus
Form: 10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide.
Applications: ICC/IF.
Immunofluorescence 1:50-1:200. The optimum dilution should be determined by the individual lab.
Storage: Store at 2-8centigrade.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Synonyms: UAE, Ulex europaeus, UAE I Lectin, Fucose lectin, UAE I, Ulex europaeus lectin, UAE Lectin
UniProt ID: P22972

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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