"Cholera Toxin A Subunit" Related Products

Native V. cholerae Cholera Toxin A Subunit

Cat.No.: CTA-114V
Product Overview: This product is the cholera toxin A subunit purified from Vibrio cholerae.
Description: Cholera toxin is the virulent factor from Vibrio cholerae that leads to severe diarrhea followed by dehydration in humans. Several bacterial toxins are ADP-ribosyltransferases with protein substrates. Many of the substrates ADP-ribosylated by bacterial protein toxins are G-proteins, which are involved in signal transduction and ADP-ribosylation is one of the more significant post translational modifications of proteins. The ADP-ribosylation activity of cholera toxin activates adenylate cyclase, resulting in the production of cyclic AMP by adenylate cyclase, which causes many metabolic alterations.
Source: V. cholerae
Species: V. cholerae
Form: Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA.
Bio-activity: ADP-ribosylation activity is measured by ADPribosylation of synthetic poly-L-arginine, following the incorporation of ADP-ribose from [U-14C] adenine NAD to TCA precipitable material. One mg of cholera toxin A subunit causes the incorporation of at least 2 pmole of ADP-ribose in 30 minutes at 30 centigrade
Molecular Mass: 27.2 kDa
Purity: ≤0.5% Cholera toxin B subunit (SDS-PAGE)
Storage: Store at 2-8 centigrade
Reconstitution: When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Synonyms: CTA; Cholera Toxin A Subunit; Cholera Toxin

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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