Description : |
This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. |
Source : |
Vicia Villosa |
Species : |
Vicia Villosa |
Form : |
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+, 0.01 mM Mn2+. |
Bio-activity : |
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
Molecular Mass : |
102 kDa - 144 kDa |
Applications : |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
Usage : |
For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml. |
Storage : |
Refrigerate; for long term storage, aliquots may be stored frozen. |
Concentration : |
2 mg/ml |
Synonyms : |
Lectin; VVL; VVA |
UniProt ID : |
P56625 |