| Description : |
This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. |
| Source : |
Vicia Villosa |
| Species : |
Vicia Villosa |
| Form : |
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+ |
| Bio-activity : |
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
| Molecular Mass : |
102 kDa - 144 kDa |
| Applications : |
Immunofluorescence, Glycobiology |
| Usage : |
The recommended concentration range for use is 5-20 µg/ml. |
| Storage : |
Refrigerate in the dark. If a precipitate forms upon long-term storage, warm to 37 centigrade. |
| Concentration : |
2 mg/ml |
| Synonyms : |
Lectin; VVL; VVA |
| UniProt ID : |
P56625 |
