Native Wisteria Floribunda Lectin Protein, Agarose bound

Cat.No.: Lectin-1868W
Product Overview: This product is the Wisteria Floribunda Lectin and and has sugar specificity against N-Acetylgalactosamine.
Description: The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Source: Wisteria floribunda
Species: Wisteria floribunda
Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 20 mM galactose, 0.08% sodium azide
Bio-activity: Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Molecular Mass: 116 kDa
Applications: Glycobiology, Affinity Chromatography
Usage: 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agaroselectin: Glycoprotein Eluting solution. Alternatively, 200 mM N-acetylgalactoseamine or several column volumes of 200 mM acetic acid can be used.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage
Storage: Refrigerate. DO NOT FREEZE.
Concentration: 3 mg/ml of settled gel
Synonyms: Lectin; WFA; WFL
UniProt ID: A0A1D5B396

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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