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Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)

Cat.No. : Kit-2195
Product Overview : Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 μU of NOS activity.
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Applications : Detection of NOS activity
Storage : -80°C
Size : 100 assays

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (4)

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In which cells is NOS present? 03/31/2022

NOS are present in neurophagocytes, endothelial cells, macrophages.

Can NOS add tags other than his? 05/05/2021

Of course, we will provide you with the most suitable tags according to your needs.

NOS is closely related to the study of which diseases? 04/03/2021

NOS is associated with abnormal vascular endothelial function, cerebral infarction and other processes.

What is the effect of lipid-lowering drugs to NOS expression? 03/01/2021

Lipid-lowering drugs can increase the expression of NOS.

Customer Reviews (3)

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02/18/2023

    The NOS proteins purchased from your company is suitable for ELISA.

    03/12/2022

      The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

      12/01/2020

        Cheap price, high quality products, worth buying again.

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